Invitro antimicrobial activities and phytochemical. Pdf antimicrobial activity of latex silver nanoparticles. Pdf antibacterial potential of calotropis procera flower extract. Antioxidant, antimicrobial activities and gcms analysis of calotropis gigantea white flowers shopna rajamohan, prabakaran kalaivanan, ilayaraja sivagnanam, manivannan rajamanickam abstract. Calotropis gigentica has shown antimicrobial activity against s. Antimicrobial activities of calotropis procera on selected. Evaluation of antibacterial activity of calotropis. Extracts on certain groups of pathogenic microorganisms.
Anti hiv1 and antimicrobial activity of the leaf extracts of calotropis. International journal of scientific and research publications, volume 7, issue 7, july 2017 keywords. Total extract proved to be an efficient antibacterial agent figure 4. Antifungal activity of leaf and latex extracts of calotropis. A bioassayguided fractionation of the crude flavonoid fraction cf3 of meoh extract which showed the highest antimicrobial activity led to the isolation of four flavonoid glycosides as the bioactive constituents.
Antimicrobial activity of endophytes from aerial and non. Biological evaluation of antimicrobial activity of calotropis. Pharmacological studies on aerial parts of calotropis procera. These same microorganisms also had their growth inhibited by some of the endophytic fungi tested in the. Antibacterial activity of aqueous extract of calotropis. Preeti juyal, vandana shrivastava, abhishek mathur subject. Asclepiadaceae and isolation of four flavonoid glycosides as the active constituents. Result of the antimicrobial activity of root extracts of calotropis gigantea. Phytochemical and antimicrobial evaluation of aqueous and. Antioxidant, antimicrobial activities and gcms analysis of calotropis gigantea white flowers shopna rajamohan, prabakaran kalaivanan, ilayaraja sivagnanam, manivannan rajamanickam abstract calotropis gigantea white asclepiadaceae, is a weed plant commonly known as giant milkweed.
The present study is related to evaluate antimicrobial activities of chloroform and methanol extracts of seeds of calotropis procera located in the forest area of ghaziabad, india. Antimicrobial activity of green silver nanoparticles from. This extract is a naturally available extract which can be used for several indications in dentistry for instance, as an antibacterial root canal obturating material or an antiseptic mouthwash. Phytochemical and pharmacological studies of the whole plant. Chloroforms extract of calotropis procera seeds exhibited better antimicrobial activity. Ocular toxicity by latex of calotropis procera sodom apple. The specific objectives 1 to determine the yield of crude extracts from leaf, root. Antioxidant, antimicrobial activities and gcms analysis. The antimicrobial activity of the aqueous, methanolic and diethyl ether extracts of the leaves, flowers and latex of c. Calotropis procera extracts against seedborne fungi agrees with the work of abdulmoniem et al. Biological activity of calotropis procera extracts 1081 introduction plants belonging to the asclepiadaceae family have a wide range of therapeutic activities. The present study was carried out to evaluate the antioxidant and antibacterial activity of leaves extracts of calotropis procera and calotropis gigantea and characterization of their bioactive metabolites by fourier transform infrared ftir spectroscopy and gas chromatographymass spectroscopy gcms. Standard antibiotic discs of kanamycin 30gdisc for bacterial species were used. The present study was carried out to evaluate the antioxidant and antibacterial activity of leaves extracts of calotropis procera and calotropis gigantea and characterization of their bioactive.
The root and latex of calotropis gigantea were screened for its antimicrobial and phytochemical activities. Approximately 675 segments from 15 calotropis procera plants. Nenaah g 20 antimicrobial activity of calotropis procera ait. Calotropis gigantea white asclepiadaceae, is a weed plant commonly known as giant milkweed. After 24 h, 50 l of calotropis procera extract was added and pictures were obtained at 0, 24 and 48 h of treatment to evaluate the antibacterial activity of calotropis procera extract. Evaluation of the effect of antimicrobial activity of.
Pdf antimicrobial activities of calotropis procera on selected. The analysis of antimicrobial activity of aqueous, methanolic and ethanolic extract of leaves and flower of calotropis procera was carried out in disc method and also determined mic value at 600nm through optical density using spectrophotometer. Antifertility activity the effect of an ethanolic extract of the roots of calotropis procera. Keywords calotropis procera, antibacterial, phytochemical, ethno medicine.
The antimicrobial effect of ethanol, aqueous and chloroform extracts of leaf and latex of calotropis procera on six bacteria namely, escherichia coli. An in vitro antimicrobial activity of calotropis procera ait. Evaluation of antibacterial activity of calotropis gigentica. Asclepiadaceae and isolation of four flavonoids glycosides as the active constituents. Antifungal, antioxidant and antibacterial activities of. Biodiversity of endophytic fungi in different leaf ages of. Phytochemical investigation of different plant parts of.
The present investigation was undertaken to confirm its claimed activity in traditional medicine. Pharmacological studies on aerial parts of calotropis procera j. Phytochemical and pharmacological studies of the whole. The bacteria tested for the sensitivity were staphylococcus aureus. Leaves, stems, fruits, roots and latex of calotropis procera extracts were evaluated for their antimicrobial activity against four types of bacteria namely staphylococcus aureus, bacillus subtilis, eschreiashia coli and pseudomonas aeruginosa. Shahzaib, abdullah khan, arif waqar zaif, wasim ahmed department of biotechnology, university of science and technology bannu, khyber pakhtunkhwa pakistan key words. Anticancer and antibacterial activity of calotropis procera. The analysis of antimicrobial activity of aqueous, methanolic and ethanolic extract of leaves and flower of calotropis procera was carried out in disc method and also determined mic value at 600nm. This extract is a naturally available extract which can be used for. Effect of latex extracts from euphorbia caducifolia and calotropis procera on rootknot nematode meloidogyne incognita infesting tomato and egg plant.
Phytochemical and antimicrobial evaluation of aqueous and organic extracts of calotropis procera ait leaf and latex. Antimicrobial activity of green silver nanoparticles from endophytic fungi isolated from calotropis procera ait latex nadia hussein mohamed 1,2, mady ahmed ismail 1, wael mostafa abdelmageed 3,4, ahmed abdelfattah mohamed shoreit 1. Phytochemical and antibacterial properties of calotropis. Antibacterial screening of crude fractions of calotropis. Antifungal, antioxidant and antibacterial activities of calotropis procera mushtaq ahmed, rehmat ali khan, s. Pdf phytochemical analysis of calotropis procera with. Agaragar was used as media for the test microorganisms. Calotropis procera has many important medicinal properties with proven pharmacological potential. All extracts were sterilized through filter sterilization using 0.
Study on antibacterial activity of calotropis procera. For antimicrobial activity testing plant latex samples were diluted by add. Disciplines, occupations and industries see more details as a purgative, anthelmintic, anticoagulant, anticancer. This study analyzed, for the first time, the community of endophytic fungi of c. Disciplines, occupations and industries see more details as a purgative, anthelmintic, anticoagulant, anticancer, antiinflammatory, antipyretic, analgesic and antimicrobial, and to treat leprosy, leucoderma, ulcers, tumours, piles, and diseases of the spleen, liver and abdomen. Antibacterial activity of crude extract and latex crude was 20. Antibacterial and phytochemical analysis of stem and root. Calotropis procera possesses antinociceptive activity, which is independent of the opioid system s aber et al.
It has one of the important traditional medicines to treat. The antimicrobial effect of ethanol, aqueous and chloroform extracts of leaf and latex of calotropis procera on six bacteria namely, escherichia coli, staphylococcus aureus, staphylococcus albus. The exudate of calotropis gigantea was tested for the antibacterial activity against a number of gram positive and gram negative bacteria. The antimicrobial activity was reported due to the presence of phenols, alkaloids, tannins and quinines 19. Biological evaluation of antimicrobial activity of.
The present study is carried out by evaluation of euphorbia hirta and calotropisprocera against mdr cultures. Many research efforts have been directed towards the provision of empirical proofs to. Antimicrobial activity of latex silver nanoparticles using calotropis procera. Antibacterial activity of calotropis procera and ficus. Evaluation of the effect of antimicrobial activity of ethanol extract of calotropis procera in extended spectrum beta lactamase producing e. Antimicrobial activity of latex was evaluated by agar disc diffusion method and inhibition zone diameters were measured according to bauer et al. The antimicrobial effect of ethanol, aqueous and chloroform extracts of leaf and latex of calotropis procera on six bacteria namely, escherichia coli, staphylococcus aureus, staphylococcus albus, streptococcus pyogenes,streptococcus pneumoniae and three fungi. Antioxidant and antibacterial activities of calotropis procera. Antifertility activity the effect of an ethanolic extract of the roots of calotropis procera has been studied in albino rats to explore its antifertility and hormonal activities. Pdf antimicrobial activity of calotropis procera ait. The antimicrobial activity of the leaf extracts of calotropis procera. Calotropis gigantea is a common wasteland weed and known for various medicinal properties.
Aspergillus niger, aspergillusflavus, microsporium boulardii and one yeast candida albicans weredetermined using agar well diffusion. Anticancer and antibacterial activity of calotropis. The plant is erect, tall, large, much branched and perennial with milky latex throughout. Antimicrobial activity and phytochemical analysis of calotropis. Antimicrobial and phytochemical screening of an ethanolic. Calotropis procera, latex, bioactive, antimicrobial activity, minimum inhibitory concentration. The decoction of the aerial part of calotropis procera is commonly used in saudi arabian traditional medicine for the treatment of variety of diseases including fever, joint pain, muscular spasm and constipation. The aim of the present study was to screen leaves of calotropis gigantea for the antimicrobial activity. Extraction and phytochemical investigation of calotropis. Antimicrobial activity of calotropis procera against proteus mirabilis different disc were prepared having concentrations of 10, 9, 5, 4. Antimicrobial activity the disc diffusion method was employed to explore the antimicrobial activity of methanolic leaf extract of c. Calotropis procera is a well known plant and has been traditionally used for the treatment of a wide range of infections globally 1720.
Phytochemical investigation of different plant parts of calotropis procera a. Calotropis procera and calotropis gigantea are medicinal plant having therapeutic value. Phytochemical analysis, antibacterial and antioxidant. Comparative study of plant extracts crude and aqueous, methanolic and ethanolic with antibiotics, provide evidence that calotropis procera extracts has the similar antibacterial activity as. Shrivastava, suchita singh, sanchita singh department of botany and biotechnology govt. Gc ms analysis and antimicrobial activity of ethanolic. Antimicrobial activity of endophytes from aerial and non aerial parts of calotropis procera against pathogenic microbes author. Calotropis gigantea crown flower is a species of calotropis the giant milkweed, calotropis procera, is native to the old world from the arabian peninsula to west, north and east africa, also from southern. Traditionally pharmacological analysis of compound s from natural or synthetic. Antimicrobial activity of extracts of jatropha curcas and calotropis procera leaves against pathogenic isolates from motorcycle helmets in lagos metropolis leonard g. Antibacterial and phytochemical screening of calotropis. Antioxidant, antimicrobial activities and gcms analysis of. Leaves, stems, fruits, roots and latex of calotropis procera extracts were evaluated for their antimicrobial activity against four types of bacteria namely staphylococcus aureus, bacillus subtilis.
Shahzaib, abdullah khan, arif waqar zaif, wasim ahmed department of biotechnology. The ethanolic extract of leaves of calotropis procera linn, had the best antimicrobial properties kareem et al. Asclepiadaceae is a small, hardy, pubescent, evergreen, erect and compact shrub which grows wild in drier and warm regions, sandy and alkaline soils in southeastern. The zone of inhibition observed was 18 mm for mrsa as shown figure 5. Antibacterial and phytochemical analysis of calotropis gigantean extract obscure function and used for human therapy 7. Antimicrobial activity of extracts and latex of calotropis.
Antimicrobial activity and phytochemical analysis of. Study on antibacterial activity of calotropis procera peerj. An in vitro antimicrobial activity of calotropis procera. The aim of the present study was to screen leaves of calotropis gigantea for the antimicrobial activity against clinical isolates of bacteria. The antimicrobial activity of the extracts of the calotropis procera, datura metel, cuscuta reflexa, pongamia pinnata and nerium oleander has been studied against the pathogenic bacteria. Invitro antimicrobial activities and phytochemical screening. Endophytes, a potential source of bioactive secondary metabolites, were isolated from the widely used medicinal plant calotropis procera ait. Antimicrobial activity of fruit latexes from ten laticiferous.
Evaluation of antimicrobial activity of euphorbia hirta and calotropis procera against mdr pathogens 1amit pandey and2nehaverma mrd life sciences pvt ltd, lucknow, 226010 abstract. Antimicrobial activity of solvent extracts and flavonoids of calotropis procera growing wild in saudi arabia was evaluated using the agar welldiffusion method. Calotropis procera roots has been reported to possess antiinflammatory activity kumar and basu, 1994. Zhang ll 2014 preparation and antimicrobial activity of tannin polymers from platycarya strobilacea infructescence. Vancomycin and oxacilline were used as positive and negative controls respectively. Antimicrobial activity and phytochemical analysis of calotropis gigantea root, latex extracts. Some of these properties may be mediated by its fungal endophytes.
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